TMT10plex™ Isobaric Label Reagents and Kits (Thermo Scientific™)

TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter. Up to ten different peptide samples prepared from cells or tissues can be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131 Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.

Key Features

The Tandem Mass Tag (TMT) Isobaric Label reagents and kits are specifically designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT10plex label reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex reagents, but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter. The different isotopes result in a 10-plex set of tags that have mass differences in the reporter that can be detected using high resolution Orbitrap MS instruments.

Advantages of the TMT10plex label reagents include increased multiplex relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents are ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or improving statistics on biological replicates.

Features of TMT10plex Isobaric Label reagent sets and mass tagging kits
Powerful—multiplexed MS analysis increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues, or biological fluids
Consistent—identical reagent structure and performance among TMTzero, TMTduplex, TMTsixplex, and TMT10plex reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
Efficient—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
Compatible—optimized for use with high resolution Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3.

Applications:
• Protein identification and quantitation from multiple samples of cells, tissue, or biological fluids
• Protein expression profiling of normal vs. disease states or control vs. treated
• Quantitative analysis of proteins for which no antibodies are available
• Identification and quantitation of membrane and post-translationally modified proteins
• Identification and quantification of hundreds to thousands of proteins in a single experiment

TMT Reagents are provided as standalone sets, a 96-well format, or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience, and reliability. When combined with the industry-leading, high resolution Thermo Scientific Orbitrap instruments and software, TMT10plex reagents provide integrated total solutions for quantitative protein expression analysis.

Order Codes

Code Description
90406 Catalog Number: 90406
Unit Size: 1 x 5 mg (per tag)
Description: TMT10plex™ Isobaric Label Reagent Set, 1 x 5 mg
90110 Catalog Number: 90110
Unit Size: 1 x 0.8 mg (per tag)
Description: TMT10plex™ Isobaric Label Reagent Set, 1 x 0.8 mg
90111 Catalog Number: 90111
Unit Size: 3 x 0.8 mg (per tag)
Description: TMT10plex™ Isobaric Label Reagent Set, 3 x 0.8 mg
90309 Catalog Number: 90309
Unit Size: 8 x 0.2 mg (per tag)
Description: TMT10plex™ Isobaric Label Reagent Set, 8 x 0.2 mg
90113 Catalog Number: 90113
Unit Size: 30 reactions
Description: TMT10plex™ Isobaric Mass Tagging Kit
A58332 Catalog Number: A58332
Unit Size: 8 x 40 μg​ (per tag)
Description: TMT™ 10plex Label Reagents ​in stabilized solution, ​96-well plate, 8 x 40 μg​
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