TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific™)

Thermo Scientific TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 μL. Each reaction yields up to 200 μg RNA from 1 μg of template in 2 hours. The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA. All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.

Highlights

• Exceptionally high yields—up to 200 μg in 2 hours
• Versatile—suitable for both short and long RNA transcripts
• Milligram amounts of RNA in a single, scaled-up reaction
• Flexible—generates unlabeled, labeled or capped RNA
• RiboRuler RNA Ladder supplied with kit for sizing and quantification

Applications

• In vitro transcription
• In vitro translation
• Generation of hybridization probes for:
• microarrays
• in situ hybridization
• blotting

• RNase protection assays
• RNA binding protein assays
• Antisense RNA and RNAi
• RNA amplification
• Microinjection studies

Includes

• TranscriptAid Enzyme Mix
• 5X TranscriptAid Reaction Buffer
• DNase I, RNase-free
• ATP Solution
• CTP Solution
• GTP Solution
• UTP Solution
• Control template
• 3 M Sodium Acetate Solution, pH 5.2
• DEPC-treated Water
• 2X RNA Loading Dye
• RiboRuler High Range RNA Ladder, ready-to-use
• 0.5 M EDTA, pH 8.0
• Detailed Protocol

* The improved version of TranscriptAid T7 High Yield Transcription Kit contains Tris-buffered NTPs instead of previously used NTPs titrated with KOH. Usage of Tris-buffered NTPs leads to higher yields of RNA transcripts.