Triton™ X-114 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Thermo Scientific Triton X-114 Surfact-Amps Detergent Solution is a stabilized 10% (w/v) solution of highly-purified Triton™ X-114 detergent, supplied in glass ampules and packaged under nitrogen inert gas, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of Triton X-114 Surfact-Amps Detergent Solution:

• Triton X-114—a nonionic detergent for use in various routine protein and molecular biology techniques
• Accurate—precise 10% detergent solution in ultrapure water
• Easy-to-use—solution is simple to dispense and dilute for use
• Exceptionally pure—less than 1.0μeq/mL peroxides and carbonyls
• Stable— packaged in glass ampules under inert nitrogen gas

Specifications for Triton X-114 Surfact-Amps Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0μeq/mL
• Carbonyls: ≤1.0μeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Properties of Triton X-114:
• Molecular Weight: 537g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: unknown
• Micelle Molecular Weight: unknown
• Critical Micelle Concentration (CMC): 0.21 mM (0.0113%, w/v)
• Cloud Point: 23°C
• Dialyzable: No

Triton X-114 is most frequently used as a component of cell lysis buffers. In certain conditions, this detergent phase-separates in aqueous solutions, a property that has been used to separate hydrophilic and hydrophobic (membrane) proteins.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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