Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.
Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 μg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.
Features of Zeba Dye and Biotin Removal columns and filter plates include:
• Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
• High recovery—low-binding resin maximizes protein recovery
• Easy-to-use—no cumbersome column preparation or equilibration
• Fast—small-molecule removal and protein recovery in less than 15 minutes
• Flexible—available in spin columns and filter spin plates for a range of needs
Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)
Available formats
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups
Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery
| Code | Description |
|---|---|
| A44296 | Catalog Number: A44296 Unit Size: 25 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL Quantity: 25 Columns |
| A44296S | Catalog Number: A44296S Unit Size: 5 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL Quantity: 5 Columns |
| A44297 | Catalog Number: A44297 Unit Size: 2 x 25 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL Quantity: 2 x 25 Columns |
| A44298 | Catalog Number: A44298 Unit Size: 5 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 2 mL Quantity: 5 Columns |
| A44299 | Catalog Number: A44299 Unit Size: 25 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 2 mL Quantity: 25 Columns |
| A44300 | Catalog Number: A44300 Unit Size: 5 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 5 mL Quantity: 5 Columns |
| A44301 | Catalog Number: A44301 Unit Size: 25 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 5 mL Quantity: 25 Columns |
| A44302 | Catalog Number: A44302 Unit Size: 5 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 10 mL Quantity: 5 Columns |
| A44303 | Catalog Number: A44303 Unit Size: 5 x 5 columns Description: Zeba™ Dye and Biotin Removal Spin Columns, 10 mL Quantity: 5 x 5 Columns |
| A44304 | Catalog Number: A44304 Unit Size: 2 plates Description: Zeba™ Dye and Biotin Removal Filter Plates, 96-well Quantity: 2 Plates |
| A44305 | Catalog Number: A44305 Unit Size: 2 x 2 plates Description: Zeba™ Dye and Biotin Removal Filter Plates, 96-well Quantity: 2 x 2 Plates |
