Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL (Thermo Scientific™)

Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Key Features

Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 μg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns and filter plates include:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 minutes
Flexible—available in spin columns and filter spin plates for a range of needs

Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)

Available formats
Spin columns: 0.5, 2, 5, and 10 mL
96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Order Codes

Code Description
A44296S Catalog Number: A44296S
Unit Size: 5 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Quantity: 5 Columns
A44296 Catalog Number: A44296
Unit Size: 25 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Quantity: 25 Columns
A44297 Catalog Number: A44297
Unit Size: 2 x 25 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Quantity: 2 x 25 Columns
A44298 Catalog Number: A44298
Unit Size: 5 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 2 mL
Quantity: 5 Columns
A44299 Catalog Number: A44299
Unit Size: 25 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 2 mL
Quantity: 25 Columns
A44300 Catalog Number: A44300
Unit Size: 5 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 5 mL
Quantity: 5 Columns
A44301 Catalog Number: A44301
Unit Size: 25 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 5 mL
Quantity: 25 Columns
A44302 Catalog Number: A44302
Unit Size: 5 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 10 mL
Quantity: 5 Columns
A44303 Catalog Number: A44303
Unit Size: 5 x 5 columns
Description: Zeba™ Dye and Biotin Removal Spin Columns, 10 mL
Quantity: 5 x 5 Columns
A44304 Catalog Number: A44304
Unit Size: 2 plates
Description: Zeba™ Dye and Biotin Removal Filter Plates, 96-well
Quantity: 2 Plates
A44305 Catalog Number: A44305
Unit Size: 2 x 2 plates
Description: Zeba™ Dye and Biotin Removal Filter Plates, 96-well
Quantity: 2 x 2 Plates
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