Zenon™ Mouse IgG1 Labeling Kits (Invitrogen™)

Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits. These antibody labeling kits are ideal for flow cytometry (e.g., FACS) experiments because they enable the simultaneous labeling of different target cells and tissues with differently labeled mouse monoclonal antibodies in a single staining protocol. Multiple Zenon™ antibody complexes may be prepared individually and used in a multicolor stain after using the Zenon™ blocking reagent.

Key Features

Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG1 labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG1 primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.

Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:
• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 μg primary antibody
• Simple—no purification required
• Flexible—choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 μg in 2 μL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.

Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.

Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.

Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.

Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.

Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

Order Codes

Code Description
Z25013 Catalog Number: Z25013
Unit Size: 1 kit
Quantity: 50 Reactions
Excitation/Emission: 402/421 nm
Label or Dye: Alexa Fluor 405
Z25002 Catalog Number: Z25002
Unit Size: 1 kit
Quantity: 50 Reactions
Excitation/Emission: 496/519 nm
Label or Dye: Alexa Fluor 488
Z25005 Catalog Number: Z25005
Unit Size: 1 kit
Quantity: 50 Reactions
Excitation/Emission: 555/565 nm
Label or Dye: Alexa Fluor 555
Z25006 Catalog Number: Z25006
Unit Size: 1 kit
Quantity: 50 Reactions
Excitation/Emission: 578/603 nm
Label or Dye: Alexa Fluor 568
Z25007 Catalog Number: Z25007
Unit Size: 1 kit
Quantity: 50 Reactions
Excitation/Emission: 590/617 nm
Label or Dye: Alexa Fluor 594
Z25008 Catalog Number: Z25008
Unit Size: 1 kit
Quantity: 50 Reactions
Excitation/Emission: 650/668 nm
Label or Dye: Alexa Fluor 647
Z25011 Catalog Number: Z25011
Unit Size: 1 kit
Quantity: 50 Reactions
Excitation/Emission: 696/719 nm
Label or Dye: Alexa Fluor 700
Z25051 Catalog Number: Z25051
Unit Size: 1 kit
Quantity: 25 Reactions
Excitation/Emission: 650/660 nm
Label or Dye: APC (Allophycocyanin)
Z25052 Catalog Number: Z25052
Unit Size: 1 kit
Quantity: 50 Reactions
Label or Dye: Biotin
Z25055 Catalog Number: Z25055
Unit Size: 1 kit
Quantity: 25 Reactions
Excitation/Emission: 496, 546, 565/578 nm
Label or Dye: R-PE (R-Phycoerythrin)
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