Zenon™ pHrodo™ iFL IgG Labeling Reagents (Invitrogen™)

Streamline antibody-drug conjugate screening campaigns with Invitrogen Zenon pHrodo IgG Labeling Reagents to screen for antibody internalization. These reagents provide rapid, scalable antibody labeling and give a bright fluorescence-based indication of antibody uptake and trafficking in target cells when analyzed by flow cytometry, fluorescence microscopy, or high-content screening.

Key Features

Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell.

The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Other advantages of the pH-sensitive Zenon™ pHrodo™ iFL IgG Labeling Reagents include:

Simplicity—No reagent purification is required
Accuracy—No wash steps or quenchers are needed, minimizing cross-reactivity or background
Speed—Labeled antibodies are typically ready to use in 10 minutes
Specificity—Dyes are designed for the specific detection of phagocytosis and endocytosis

Preserve primary antibody function and affinities
Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes. One of these is reduced binding affinity due to label addition in the binding pocket. The Zenon™ antibody labeling approach, which is targeted to the Fc tail, resolves this potential issue.

Try custom antibody conjugates
If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Order Codes

Code Description
Z25612 Catalog Number: Z25612
Unit Size: 240 µL
Species: Human
Labeling Target: IgG
Description: pHrodo™ iFL Red, 240 μL
Color: Red
Z25609 Catalog Number: Z25609
Unit Size: 240 µL
Species: Mouse
Labeling Target: IgG
Description: pHrodo™ iFL Green, 240 μL
Color: Green
Z25618 Catalog Number: Z25618
Unit Size: 240 μL
Species: Human
Labeling Target: IgG
Description: pHrodo™ Deep Red, 240 μL
Color: Deep Red
Z25610 Catalog Number: Z25610
Unit Size: 240 µL
Species: Mouse
Labeling Target: IgG
Description: pHrodo™ iFL Red, 240 μL
Color: Red
Z25622 Catalog Number: Z25622
Unit Size: 240 μL
Species: Mouse
Labeling Target: IgG
Description: pHrodo™ Deep Red, 240 μL
Color: Deep Red
Z25611 Catalog Number: Z25611
Unit Size: 240 µL
Species: Human
Labeling Target: IgG
Description: pHrodo™ iFL Green, 240 μL
Color: Green
Z25613 Catalog Number: Z25613
Unit Size: 1.2 mL
Species: Human
Labeling Target: IgG
Description: pHrodo™ iFL Green, 1.2 mL
Color: Green
Z25614 Catalog Number: Z25614
Unit Size: 1.2 mL
Species: Human
Labeling Target: IgG
Description: pHrodo™ iFL Red, 1.2 mL
Color: Red
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