Griffonia Simplicifolia (African Legume) Lectin I (GSL I), DyLight 594 (Invitrogen™)

Griffonia Simplicifolia Lectin I (GSL I-B4), DyLight 594, is a bright red GSL I-B4-fluorophor conjugate that is sourced from the African legume Griffonia simplicifolia (Bandeiraea simplicifolia). This B4 isoform of GSL I contains only B subunits and is widely used as a marker of endothelial cells in non-primates such as mouse, rat, rabbit, and goat, as well as a marker of non-peptidergic unmyelinated primary afferent neurons.

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• Very bright DyLight 594 conjugate detected with traditional Texas Red filter set, Ex/Em: 592/617 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: galactose
• Inhibiting/eluting sugar: 500 mM galactose (S-9003) or 100 mM raffinose
• Blood group specificity: B>>A1
• Unconjugated lectin molecular weight: 114 KDa
• Immunofluorescence working concentration: 5–20 μg/mL

GSL I-B4-DyLight 594 is provided at a 1 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca²⁺. If a precipitate forms upon long-term storage, warm to 37°C.

GSL I is a tetrameric lectin consisting of A and B subunits. There are five GSL-I isolectins with different subunit compositions. The B4 isolectin (all B subunits) is blood-group-B specific and has an exclusive affinity for terminal α-D-galactosyl residues. It specifically agglutinates blood group B erythrocytes and was originally employed for this purpose. Subsequent work has shown that the GSL I B4 isolectin is cytotoxic to several normal and tumor cell types and has particularly strong affinity for brain microglial and perivascular cells. It has also been particularly valuable as a histochemical and flow cytometric probe for specifically labeling endothelial cells in a number of species. GSL I B4 isolectin has been used effectively for tracing central and peripheral neuronal pathways following local injections, as well as for labeling stimulated murine macrophages, bovine thyroid cells, various murine cell types, laminin, and thyroglobulin.