Poly(A) Polymerase (cloned) 2 U/μL (Invitrogen™)

Ambion™ Poly(A) Polymerase catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion. The enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs. One tube containing 80 U (2 U/ μL), 5X Poly(A) Polymerase Buffer, and 25 mM MnCl₂ are provided. The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence.

Unit Definition:
One unit is the amount of enzyme required to incorporate 1 nmol of AMP into acid-insoluble material in 10 min at 37°C.