Emission filters for Thermo Scientific Varioskan™ LUX Multimode Microplate Readers (Thermo Scientific™)

Varioskan LUX comes equipped with a range of measurement technologies including absorbance and fluorescence intensity with optional luminescence, AlphaScreen and TRF. The instrument selects the measurement wavelength using monochromators or optical filters, depending on which is optimal for each measurement technology. A wide range of optional filters extends the application area remarkably, including luciferase assays, Bioluminescence Resonance Energy Transfer (BRET) assays, Homogeneous Time Resolved Fluorescence (HTRF) assays and many other.

Key Features

Extensive range of different type of emission filters are available for various applications when performing luminometric, TRF or Alpha technology assays, such as TR-FRET, hTRF, BRET, NanoBRET, AlphaScreen/AlphaLisa and all multiplexing assays using these detection technologies.
Excitation filters for TRF and AlphaScreen/AlphaLisa measurements are permanently fixed inside the corresponding detection technology optical module.
Emission filter installation is effortless. The instrument filter wheel is easy to access, and the correct parameters are quickly set in simple workflow in Thermo Scientific SkanIt software. SkanIt software controlled installation process also prevents accidental mistakes during the process and therefore ensures proper operation.
In most luminescence measurements, there is only one source of light in the system and therefore the most efficient detection is received by collecting all the light emitted by the sample, independent on the wavelength. Therefore, these assays do not require any wavelength selection. However, certain in applications, like BRET or any luminometric multiplexing assay, where multiple light sources are present in the sample, filters are used to collect the light produced at specific wavelength.
All Varioskan LUX filters can be used for any filter based detection technology (luminescence, TRF or Alpha technology), depending on the application. Please contact technical support for more information concerning your specific application requirements.

Order Codes

Code	Description
F49210	Catalog Number: F49210 Unit Size: Each Product Type: Emission Filter Wavelength: 492 nm For Use With (Application): General Terbium measurement
F46080	Catalog Number: F46080 Unit Size: Each Product Type: Emission Filter Wavelength: 460 nm For Use With (Application): NanoBRET donor
F48020	Catalog Number: F48020 Unit Size: Each Product Type: Emission Filter Wavelength: 480 nm For Use With (Application): BRET donor using wtRluc
F51060	Catalog Number: F51060 Unit Size: Each Product Type: Emission Filter Wavelength: 510 nm For Use With (Application): Chroma-glo luciferase CBG68 or CBG99 assay
F51530	Catalog Number: F51530 Unit Size: Each Product Type: Emission Filter Wavelength: 515 nm For Use With (Application): BRET2 acceptor using GFP2
F52010	Catalog Number: F52010 Unit Size: Each Product Type: Emission Filter Wavelength: 520 nm For Use With (Application): LanthaScreen measurement of Tb acceptor; HTRF donor measurement of TbGreen
F52530	Catalog Number: F52530 Unit Size: Each Product Type: Emission Filter Wavelength: 525 nm For Use With (Application): Pierce green Renilla luciferase assay
F53030	Catalog Number: F53030 Unit Size: Each Product Type: Emission Filter Wavelength: 530 nm For Use With (Application): BRET acceptor using eYFP
F57177	Catalog Number: F57177 Unit Size: Each Product Type: Emission Filter Wavelength: 571 nm For Use With (Application): AlphaScreen measurement
F61060	Catalog Number: F61060 Unit Size: Each Product Type: Emission Filter Wavelength: 610 nm For Use With (Application): Chroma-glo luciferase CBR assay
F610LP	Catalog Number: F610LP Unit Size: Each Product Type: Emission Filter Wavelength: 610 nm For Use With (Application): NanoBRET acceptor
F61520	Catalog Number: F61520 Unit Size: Each Product Type: Emission Filter Wavelength: 615 nm For Use With (Application): AlphaLisa measurement
F62010	Catalog Number: F62010 Unit Size: Each Product Type: Emission Filter Wavelength: 620 nm For Use With (Application): General Europium measurement; LanthaScreen measurement of Eu donor; HTRF acceptor measurement of TbGreen; HTRF donor measurement of EuRed and TbRed
F640LP	Catalog Number: F640LP Unit Size: Each Product Type: Emission Filter Wavelength: 640 nm For Use With (Application): Pierce red firefly luciferase assay
F66510	Catalog Number: F66510 Unit Size: Each Product Type: Emission Filter Wavelength: 665 nm For Use With (Application): LanthaScreen measurement of Eu acceptor; HTRF acceptor measurement of EuRed and TbRed
F47040	Catalog Number: F47040 Unit Size: Each Product Type: 470/ 40 nm emission filter Wavelength: 470 nm For Use With (Application): Pierce Cypridina and Gaussia luciferase assays