iBlot™ 3 iBind™ Flex Western Starter Kit (Invitrogen™)

The iBlot 3 iBind Flex Western Starter Kit includes the iBlot 3 Western Blot Transfer Device, the iBind Flex Western Device, and all of the consumables and reagents necessary to get started using both devices for fast, efficient transfer and western processing.

Contents of the iBlot 3 iBind Flex Western Starter Kit:
• iBlot 3 Western Blot Transfer Device
• iBlot 3 Transfer Stacks, Midi, NC (nitrocellulose)
• iBind Flex Western Device
• iBind Flex Cards
• iBind Flex Solution Kit
• SuperSignal West Pico Plus Chemiluminescent Substrate

iBlot 3 Western Blot Transfer Device
The iBlot 3 Western Blot Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably in as few as three minutes and without the need for liquid buffers. The iBlot 3 system is compatible with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes and offers equivalent or better performance compared with traditional wet-transfer methods in a fraction of the time. The iBlot 3 Western Blot Transfer Device is an integral part of the iBlot dry blotting system, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

iBind Flex Western Device
The iBind Flex Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays. Each solution is released from the iBind Flex device wells to an iBind Flex Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

The iBind Flex western detection procedure minimizes hands-on time and is highly reproducible. Setup time is fast, and each solution is released from the iBind Flex device wells to an iBind Flex Card via SLF in ∼2.5 hours. Compared to manual methods, the iBind Flex western system requires less primary antibody, enables reproducible results, and is compatible with downstream detection protocols. The iBind Flex Western Device includes three user-interchangeable well inserts to process up to one midi blot, two mini blots, or six vertically-cut strip blots.