iBlot™ 3 iBind™ Western Starter Kit (Invitrogen™)

The iBlot 3 iBind Western Starter Kit includes the iBlot 3 Western Blot Transfer Device, the iBind Western Device, and all of the consumables and reagents necessary to get started using both devices for fast, efficient transfer and western processing.

Contents of the iBlot 3 iBind Western Starter Kit:
• iBlot 3 Western Blot Transfer Device
• iBlot 3 Transfer Stacks, Midi, NC (nitrocellulose)
• iBind Western Device
• iBind Cards
• iBind Solution Kit
• SuperSignal West Pico Plus Chemiluminescent Substrate

iBlot 3 Western Blot Transfer Device
The iBlot 3 Western Blot Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably in as few as three minutes and without the need for liquid buffers. The iBlot 3 system is compatible with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes and offers equivalent or better performance compared with traditional wet-transfer methods in a fraction of the time. The iBlot 3 Western Blot Transfer Device is an integral part of the iBlot dry blotting system, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

iBind Western Device
The iBind Western Device is an automated western-processing device that performs every step in the western protocol from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays. Each solution is released from the iBind device wells to an iBind Card via SLF in ∼2.5 hours. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction. The iBind western detection procedure minimizes hands-on time and is highly reproducible and compatible with all western protocols.