iBlot™ 3 Transfer Stacks, midi, PVDF (Invitrogen™)

iBlot 3 Transfer Stacks are used with iBlot 3 Western Blot Transfer Device for the transfer of proteins from an SDS-PAGE gel to a membrane support when performing western blot experiments. Each iBlot 3 Transfer Stack contains copper-fiber electrode sheets, optimized cathode and anode solid buffer gels, and a preconditioned PVDF transfer membrane. This midi-size stack (13 cm x 8.3 cm) can be used with midi-size gels or two mini-size gels.

The iBlot 3 Western Blot Transfer System (combined iBlot 3 Transfer Stacks and iBlot3 Transfer Device) have been optimized to provide protein transfers in 3–8 minutes that produce higher quality and more reliable western blot result compared to many other transfer methods.

PVDF membrane
The PVDF membrane (0.2-μm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. Proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 μg/cm².