Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.2-μm pore size is well-suited for western blotting, and this smaller pore size is ideal for the transfer of low molecular weight proteins or peptides and nucleic acids (<300 bp).
Features include:
100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background—produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting
Code | Description |
---|---|
77012 | Catalog Number: 77012 Unit Size: 25 pre-cut membranes Dimensions (LxW): 12 x 8 cm Format: Sheet Pore Size: 0.2 μm Sufficient For: 25 Mini-gel Blots |
88013 | Catalog Number: 88013 Unit Size: 15 sheets Dimensions (LxW): 10.5 x 7.9 cm Format: Sheet Pore Size: 0.2 μm Sufficient For: 15 Mini-gel Blots |
88024 | Catalog Number: 88024 Unit Size: 15 pre-cut membranes Dimensions (LxW): 8 x 8 cm Format: Sheet Pore Size: 0.2 μm Sufficient For: 15 Mini-gel Blots |