Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.45-μm pore size is well-suited for a wide range of western blotting conditions and ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).
Features include:
• 100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets or choose the roll format for the flexibility to cut to the ideal size
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background: produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting
Code | Description |
---|---|
88018 | Catalog Number: 88018 Unit Size: 1 roll Dimensions (LxW): 3.5 m x 30 cm Format: Roll Pore Size: 0.45 μm Sufficient For: ≥120 Mini-gel Blots (When cut to 8 x 10 cm) |
88025 | Catalog Number: 88025 Unit Size: 15 pre-cut membranes Dimensions (LxW): 8 x 8 cm Format: Sheet Pore Size: 0.45 μm Sufficient For: 15 Mini-gel Blots |
88014 | Catalog Number: 88014 Unit Size: 15 sheets Dimensions (LxW): 10.5 x 7.9 cm Format: Sheet Pore Size: 0.45 μm Sufficient For: 15 Mini-gel Blots |
77010 | Catalog Number: 77010 Unit Size: 25 pre-cut membranes Dimensions (LxW): 12 x 8 cm Format: Sheet Pore Size: 0.45 μm Sufficient For: 25 Mini-gel Blots |