Pierce™ 1-Step Transfer Buffer (Thermo Scientific™)

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Power Blotter.

Features of 1-Step Transfer Buffer:

• Fast—high ionic strength formulation provides 5- to 10-minute protein transfer when used with compatible fast semi-dry blotting systems
• Ready to use—supplied as a 1X solution that is stable and ready to use at room temperature
• Optimized—designed for seamless and reliable performance with the Power Blotter
• Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217)
• Economical—excellent performance without the special or costly consumables that are required by other fast semi-dry transfer devices

When used with the Power Blotter, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 10 minutes with efficiency that is equal to, or better than, conventional Western blotting techniques. Pierce 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217), when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.

Requires:
Power Blotter (Part No. 62288) or other semi-dry transfer device that is equipped or paired with a capable high-current power supply.

Fast blotting methods require a high-current power supply, such as the Power Blotter, and an optimized high ionic strength transfer buffer, such as Pierce 1-Step Transfer Buffer. By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (∼22-23 mA/cm2) and voltage is limited to 25 V.