Pierce™ Protein-Free Blocking Buffer (Thermo Scientific™)
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.
Key Features
Features of Protein-Free Blocking Buffer include:
• Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
• Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
• Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
• High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.
Order Codes
Code |
Description |
37570 |
Catalog Number: 37570 Unit Size: 1 L Description: Protein-Free (TBS) Blocking Buffer, 1 L |
37584 |
Catalog Number: 37584 Unit Size: 100 mL Description: Protein-Free (PBS) Blocking Buffer, 100 mL |
37572 |
Catalog Number: 37572 Unit Size: 1 L Description: Protein-Free (PBS) Blocking Buffer, 1 L |
37585 |
Catalog Number: 37585 Unit Size: 100 mL Description: Protein-Free (TBS) Blocking Buffer, 100 mL |
37573 |
Catalog Number: 37573 Unit Size: 1 L Description: Protein-Free T20 (PBS) Blocking Buffer, 1 L |
37571 |
Catalog Number: 37571 Unit Size: 1 L Description: Protein-Free T20 (TBS) Blocking Buffer, 1 L |