Pierce™ Protein-Free Blocking Buffer (Thermo Scientific™)

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

Key Features

Features of Protein-Free Blocking Buffer include:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Order Codes

Code Description
37570 Catalog Number: 37570
Unit Size: 1 L
Description: Protein-Free (TBS) Blocking Buffer, 1 L
37584 Catalog Number: 37584
Unit Size: 100 mL
Description: Protein-Free (PBS) Blocking Buffer, 100 mL
37572 Catalog Number: 37572
Unit Size: 1 L
Description: Protein-Free (PBS) Blocking Buffer, 1 L
37585 Catalog Number: 37585
Unit Size: 100 mL
Description: Protein-Free (TBS) Blocking Buffer, 100 mL
37573 Catalog Number: 37573
Unit Size: 1 L
Description: Protein-Free T20 (PBS) Blocking Buffer, 1 L
37571 Catalog Number: 37571
Unit Size: 1 L
Description: Protein-Free T20 (TBS) Blocking Buffer, 1 L
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