Pierce™ Protein-Free Blocking Buffer (Thermo Scientific™)
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.
Key Features
Features of Protein-Free Blocking Buffer include:
• Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
• Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
• Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
• High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.
Order Codes
| Code |
Description |
| 37584 |
Catalog Number: 37584
Unit Size: 100 mL
Description: Protein-Free (PBS) Blocking Buffer, 100 mL |
| 37572 |
Catalog Number: 37572
Unit Size: 1 L
Description: Protein-Free (PBS) Blocking Buffer, 1 L |
| 37585 |
Catalog Number: 37585
Unit Size: 100 mL
Description: Protein-Free (TBS) Blocking Buffer, 100 mL |
| 37570 |
Catalog Number: 37570
Unit Size: 1 L
Description: Protein-Free (TBS) Blocking Buffer, 1 L |
| 37573 |
Catalog Number: 37573
Unit Size: 1 L
Description: Protein-Free T20 (PBS) Blocking Buffer, 1 L |
| 37571 |
Catalog Number: 37571
Unit Size: 1 L
Description: Protein-Free T20 (TBS) Blocking Buffer, 1 L |
